Plasmid

Part:BBa_K4739013:Design

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-11)


Knockout plasmid for exoS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal PstI site found at 1195
    Illegal PstI site found at 1795
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal NheI site found at 3743
    Illegal PstI site found at 1195
    Illegal PstI site found at 1795
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal BglII site found at 1779
    Illegal BglII site found at 4300
    Illegal BamHI site found at 821
    Illegal XhoI site found at 16
    Illegal XhoI site found at 1560
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal PstI site found at 1195
    Illegal PstI site found at 1795
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal PstI site found at 1195
    Illegal PstI site found at 1795
    Illegal NgoMIV site found at 311
    Illegal NgoMIV site found at 428
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 1569
    Illegal NgoMIV site found at 4636
    Illegal NgoMIV site found at 4646
    Illegal AgeI site found at 886
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1444
    Illegal SapI.rc site found at 5914


Design Notes

The melting temperature and GC content of KO primer.


Source

The sequence of the homologous arm of exoS is from the genome of P. aeruginosa assembled through Gibson assembly.

References